KMID : 0869020060090010040
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Journal of Korean Orthopaedic Research Society 2006 Volume.9 No. 1 p.40 ~ p.47
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Role of Tumor Necrosis Factor Alpha, Interleukin 8 and Dexamethasone in the FAK Expression by Human Nucleus Pulposus Cells
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Yoon Jin-Ho
Lee Ju-Hong
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Abstract
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Purpose: Human nucleus pulposus cells from intervertebral disc specimens were cultured to study the effects of TNF-alphaand IL-8 on the FAK expression by these cells. The effect of co-stimulation with dexamethasone on the FAK expression by nucleus pulposus cells was also studied.
Materials and Methods:The FAK expression in cultured human nucleus pulposus cells relation to TNF-alpha, IL-8 and dexamethasone was assessed by performing western blot.
Results:The activated FAK expression was up-regulated by treatments of TNF-alpha and IL-8. Dexamethasone attenuated the cytokine-induced FAK expression. The effects of inflammatory cytokines on the FAK expression were found to be concentration dependent with greater correlation shown by IL-8 than by TNF-alpha.
Conclusion:The FAK expression of human nucleus pulposus cells was up-regulated by TNF-alpha and IL-8 stimulation and it was attenuated by the co-administration of dexamethasone.
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KEYWORD
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Human uncleus pulposus, Focal adhesion kinase, Tumor necrosis factor alpha, Interleukin-8
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